What is the DOT Urine Sample Testing Protocol?

December 20, 2024

Ophthalmologist's Department

The Department of Transportation (DOT) ensures safety across the transportation industry. A cornerstone of these efforts is the drug testing process, which includes a detailed urine sample testing protocol. This procedure is designed to maintain integrity, accuracy, and fairness while ensuring the safety of our roadways and work environments. Here’s a breakdown of the protocol:

1. Collection Site

Urine samples must be collected at an approved and designated collection site. These can include physician offices, commercial collection facilities, or local hospitals. The site must adhere to DOT guidelines to ensure a secure and controlled environment for testing.

2. Specimen Collection

The individual provides a urine sample in a private area, with direct observation implemented in specific cases to prevent tampering or adulteration. This step ensures the authenticity of the sample while respecting the individual’s privacy whenever possible.

3. Custody and Control

Once collected, the urine sample is immediately sealed, labeled, and accompanied by a Custody and Control Form (CCF). This document is essential for tracking the sample from collection through testing and ensures that the chain of custody remains intact at every stage.

4. Laboratory Testing

The sealed sample is sent to a certified laboratory, where it is tested for the presence of five specific substances:

  • Marijuana (THC)
  • Cocaine
  • Opiates (e.g., codeine, morphine)
  • Amphetamines (e.g., methamphetamine)
  • Phencyclidine (PCP)

These substances are known to impair performance and pose safety risks, making them a priority for DOT testing.

5. Medical Review Officer (MRO) Review

If the laboratory detects a positive result, the Medical Review Officer (MRO) steps in to review the findings. The MRO contacts the individual to discuss potential medical explanations for the result, such as prescription medications. This step ensures a fair assessment before the results are finalized.

6. Reporting Results

Once the review is complete, the final results are reported to the employer. Depending on the outcome, the employer determines the appropriate course of action, such as further evaluation or removal from safety-sensitive duties.

Why is the Protocol Important?

The DOT urine sample testing protocol is an important tool for maintaining a drug-free workplace in the transportation industry. By following strict guidelines, the protocol ensures fairness, accuracy, and accountability while promoting safety for workers and the public.

For more information about DOT drug testing requirements and how they impact your business, visit Resilient Return. You can also book a DOT SAP program appointment with us.

Karishma Sarfani

Karishma is a Qualified Substance Abuse Professional (SAP) with a background in mental health and substance abuse counseling, holding credentials as an MS, LCDC, ICADC, CADC II, CSAC, CASAC II, LPC, and EMDR-trained therapist. Inspired by personal experiences with addiction and mental health challenges in her community, she has dedicated her career to supporting individuals on their journey to recovery and success.